Isolation, screening and characterization of phosphate solubilizing bacteria from karwar costal region. An aliquot 100l from decimal dilutions was inoculated on picovskayas medium by pour plate technique and incubated at 30oc. Isolation and biological characterization of phosphate. Nagalakshmi department of microbiology, kanchi shri krishna college of arts and science, kilambi, kanchipuram631551, tn, india corresponding author abstract introduction phosphorus p is a major growthlimiting. Abid ullah 1, hafsa mushtaq 2, usman ali 1, hakim 1, ehsan ali 3, samavia mubeen2 1department of plant science and technology, huazhong agricultural university, wuhan, hubei, p. Nitrogen fixing and phosphate solubilizing bacteria can be isolated from mining area soil. Agronomy free fulltext isolation and characterization.
Coinoculation of phosphate solubilizing bacteria and rhizobia in presence of ltryptophan for the promotion of mash bean vigna mungo l. Among them about 78 isolates was found from the different rhizosphere of sunflower plants. Ghoulam 1, 1 unit of biotechnology and symbioses agrophysiology, sciences and techniques faculty, po. Three isolates selected between the 18 isolated strains according to their ability to hydrolyze organic phosphate.
Characterization of phosphatesolubilizing bacteria isolated from acidic ultisol soil, south borneo to cite this article. Sinha, isolation and characterization of phosphate solubilizing bacterium pseudomonas. Isolation and characterization of phosphate solubilizing bacteria from cultivated fields of south and north gondar zones of western ethiopia mokula mohammed raffi1, melese yigzaw2 and muluken dejen3 1,2department of natural resource management, college of agriculture and rural transformation. The objective of this research was to isolate phosphate solubilizing bacteria psb from faba bean rhizosphere in meknes region. Pdf isolation and characterization of phosphate solubilizing. Jasmine, onion, phosphate, phosphate solubilizing bacteria, rhizosphere, random amplified polymorphic dna how to cite this article.
Isolation, characterization, and molecular identification of phosphate solubilizing bacteria from several tropical soils. Isolation and characterization of phosphatesolubilizing bacterial species from different crop fields of. Psb could convert these insoluble phosphates into available forms for plant via acidification, chelation, exchange reactions, and production of gluconic isolation and characterization of phosphatesolubilizing bacteria. The population level of psb was higher in the rhizosphere soils collected from lemon followed by. These bacterial strains were identified by sequence analysis of 16s rrna genes as bacterial species of bacillus megaterium b. Isolation and identification of temperature tolerant phosphate solubilizing bacteria ttpsb and their use as microbial fertilizers was the main goal of the study. Isolation and characterization of phosphate solubilizing bacteria from rhizosphere soil. Isolation and characterization of ralstonia pickettii a.
The objectives of this work were to isolate and characterize walnut phosphate solubilizing bacteria psb and to evaluate the effect of inoculation with the selected psb stains to walnut seedlings fertilized with or without insoluble phosphate. Isolation and characterization of phosphate solubilizing bacteria from rhizosphere of coffee plant and. Isolation, characterization and interrelationship of phosphate solubilizing bacteria from the rhizosphere of sugarcane and rice author links open overlay panel muhammad awais a b muhammad tariq a arfan ali a b qurban ali a anwar khan a bushra tabassum a idrees ahmad nasir a tayyab husnain a. Phosphate solubilizing bacteria are frequently used as plant growth promoters. Isolation and characterization of phosphate solubilizing bacteria from cultivated fields of south and north gondar zones of western ethiopia mokula mohammed raffi1, melese yigzaw2 and muluken dejen3 1,2department of natural resource management, college of. Isolation and phenotypic characterization of phosphate solubilizing bacteria from lentil lens culnaris. Isolation and characterization of phosphate solubilizing bacterium pseudomonas aeruginosa kupsb12 with antibacterial potential from river ganga, india dipak paul, sankar narayan sinha environmental microbiology research laboratory, department of botany, university of kalyani, kalyani 741235, west bengal, india article info article history. The objectives of this work were to isolate and characterize walnut phosphatesolubilizing bacteria psb and to evaluate the effect of inoculation with the selected psb stains to walnut seedlings fertilized with or without insoluble phosphate. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. Isolation and characterization of phosphate solubilizing rhizo bacteria to improve plant health of tomato j. Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Highly efficient phosphatesolubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. Isolation and phenotypic characterization of phosphate. Frontiers isolation and characterization of a phosphorus.
In this study 50 collected soil samples were screened on the purpose of isolating psb. Isolation, characterization and interrelationship of phosphate solubilizing bacteria from the rhizosphere of sugarcane and rice. Phosphate solubilizing bacteria psb were selected based on the quantitative ability to solubilize. One gram 1 g of each soil sample was dispersed in 9 ml of autoclaved distilled water, filtered by 125 mm whatman. Wang z, xu g, ma p, lin y, yang x and cao c 2017 isolation and characterization of a phosphorus solubilizing bacterium from rhizosphere soils and its colonization of chinese cabbage brassica campestris ssp. The direct plant growth promotion is done by the production of indole acetic acid iaa. Isolation of phosphate solubilizing bacteria from maize rhizosphere and their potential for rock phosphate solubilizationmineralization and plant growth promotion. Identification and characterization of the phosphate. Isolation and characterization of ralstonia pickettiia. Twenty phosphatesolubilizing bacteria psb were isolated from calcareous rhizosphere soils. Phosphatesolubilizing bacteria are frequently used as plant growth promoters. Isolation and partial characterization of phosphate solubilizing bacteria isolated from soil and marine samples talat yasmeen mujahid 1, khaizran siddiqui 2, rifat ahmed 2, shahana.
Isolation and characterization of phosphate solubilizing bacteria from chinese cabbage sungman woo, minkyoung lee, insoo hong, s. Isolation and characterization of phosphate solubilizing bacteria. Isolation, characterization, and growth promotion of phosphate solubilizing bacteria associated with nicotiana tabacum tobacco lin gao1, fanyu kong1, chao feng1, jing wang1, jiaming gao2, guoming shen1, chengsheng zhang1 1tobacco research institute of chinese academy of agricultural sciences, qingdao 266101, pr china. Twenty phosphate solubilizing bacteria psb were isolated from calcareous rhizosphere soils. Colonies showing phosphate solubilizing zone around them were considered as psm. Original research isolation, characterization, and growth. The population level of psb was higher in the rhizosphere soils collected from lemon followed by jack fruit. Wang z, xu g, ma p, lin y, yang x and cao c 2017 isolation and characterization of a phosphorussolubilizing bacterium from rhizosphere soils and its colonization of chinese cabbage brassica campestris ssp. Isolation and characterization of the high silicate and phosphate solubilizing novel strain enterobacter ludwigii gak2 that promotes growth in rice plants by koeun lee 1, arjun adhikari 1, sangmo kang 1, younghyun you 2, giljae joo 3, jinho kim 4, sangjun kim 5 and injung lee 1. Isolation and characterization of phosphate solubilizing. We used 96well microplates instead of traditional 200mm petri dishes to rapidly screen ipsb strains for their solubilizing ability. Jasmine, onion, phosphate, phosphatesolubilizing bacteria, rhizosphere, random amplified polymorphic dna how to cite this article.
Abstract phosphatesolubilizing bacterial strains 6 nos. Phosphate solubilizing bacteria psb are soil bacteria, capable of turning insoluble form of phosphorus into the usable component for plants. Pdf on jan 20, 2018, deepak pandey and others published isolation and characterization of phosphate solubilizing bacteria from the. Isolation and characterization of phosphate solubilizing bacteria from phosphate solid sludge of the moroccan phosphate mines fatima z. Submitted 24 december 2015, revised 29 march 2016, accepted 12 april 2016. Aug 25, 2018 phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. Isolation, identification and characterization of phosphate solubilizing bacteria psb isolated from economically important crop plants n. Phosphorus solubilizing bacteria psb play important role by enhancing its availability. Isolation and characterization of phosphatesolubilizing bacteria from rhizosphere soil. Phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. Guru nanak institute of research and development, guru nanak khalsa college, nathalal parekh marg, matunga e, mumbai, maharashtra, india.
Identification and characterization of inorganicphosphate. Here we report isolation of ralstonia pickettii a novel phosphate solubilizing bacterium from a. Pdf isolation and partial characterization of phosphate. However, the characterization of different organic acids produced by the. In this regard, the ability of phosphofungi to dissolve insoluble phosphorus compounds is greater than that of phosphatesolubilizing bacteria. The present investigation is aimed at to isolation, identification and characterization of phosphate solubilizing bacteria from the economically important tree species such as lemon, jackfruit and mango. Poonguzhali and tongmin sa department of agricultural chemistry, chungbuk national university, cheongju, chungbuk, 361763, republic of korea. In the field of agricultural microbiology attempts are being made to find novel microbes for use as efficient biofertilizers including phosphate solubilizing microbes psm. Rhizospheric microorganisms can increase phosphorus availability in the soil. Isolation of phosphate solubilizing bacterial strain phosphate solubilizing bacterial strain isolated from a jute mill effluent mixing area of ganga river water at bansberia 2258. China 2department of plant sciences, faculty of biological sciences, quaidiazam university, islamabad, pakistan. The objective of this research was to isolate phosphate solubilizing bacteria psb from faba bean rhizosphere in meknes region and evaluate in vitro their potential for p solubilization as well as their response to the effect of temperature. A study was conducted to isolate phosphate solubilizing bacteria psb from aerobic rice grown in penang malaysia and to determine some biochemical properties of the isolates such as, organic acids, enzymes, indoleacetic acid iaa, siderophore production and its antagonistic effect against pathogen rhizoctonia solani. Isolation and screening of phosphate solubilizing bacteria wide number of bacterial isolates was found from the different agricultural lands of surat and bharuch district in gujarat, india.
For isolation of phosphate solubilizing microbes, 1g rhizosphere soil was suspended in 100ml of distilled water. Isolation, screening and characterization of phosphate. Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of faba bean vicia faba l. Jemeema begum department of botany, ayya nadar janaki ammal college, sivakasi 626 125, tamil nadu, india corresponding author abstract introduction. Isolation of phosphate solubilizing rhizobacteria was carried out by spread plate technique. Characterization of phosphatesolubilizing bacteria. Isolation, characterization, and growth promotion of phosphatesolubilizing bacteria associated with nicotiana tabacum tobacco lin gao1, fanyu kong1, chao feng1, jing wang1, jiaming gao2, guoming shen1, chengsheng zhang1 1tobacco research institute of chinese academy of agricultural sciences, qingdao 266101, pr china. Characterization of phosphatesolubilizing bacteria isolated. Isolation, characterization, and molecular identification. Isolation and characterization of phosphate solubilizing rhizobia bacteria nodulating vicia faba l. Thirtyfour psb strains were isolated and identified under the genera pseudomonas, stenotrophomonas, bacillus, cupriavidus, agrobacterium. Isolation and characterization of one strain of phosphate.
Psb could convert these insoluble phosphates into available forms for plant via acidification, chelation, exchange reactions, and production of gluconic isolation and characterization of phosphate solubilizing bacteria. Isolation and characterization of phosphofungi, and. Abstract phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. More p can be released by inoculating soil with inorganicphosphatesolubilizing bacteria ipsbs. The ability to solubilize fixed inorganic phosphorus p for plant growth is important for increasing crop yield. Isolation, characterization and interrelationship of. Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil. Paper open access related content characterization and. Isolation of phosphate solubilizing bacteria bacterial isolation was carried out from each soil sample after homogenously suspending in sterile saline solution 0. Particularly, research on use of microbes for improving availability of phosphorus continues to be a major area of focus. Rhizosphere soils from southern parts of tigray, ethiopia adigo setargie, sefinew tilahun, samuel alemayehu, tadesse dejenie and solomon kiros department of biology, college of natural and computational sciences, mekelle university, ethiopia. Isolation and characterization of a phosphatesolubilizing.
Phenotypic and genotypic characterization of phosphate solubilizing. Isolation, identification and characterization of phosphate. Isolation and screening of p solubilizing bacteria the soil adhering to the roots of aloe barbadensis was separated by gentle tapping, using sterilized forceps and collected in sterilized plastic bags. Phosphate solubilizing bacteria psb play an important. Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Agronomy free fulltext isolation and characterization of. Plant growth promotion induced by phosphate solubilizing endophytic pseudomonas isolates. Isolation and characterization of phosphate solubilizing bacterial species from different crop fields of salem, tamil nadu, india. Dilutions of soil samples were prepared of the root homogenate and soil.
Materials and methods isolation of phosphate solubilizing bacterial strains the strain was isolated from heavy metal contaminated soil collected from abandoned mines at boryeong area in south korea. Aliquots of 106 diluted samples serial dilutions were spread on pikovskayas agar medium pikovskaya, 1948 and. Isolation and characterization of phosphatesolubilizing bacteria. Phosphate solubilizing bacteria provide an ecofriendly alternative to convert. Five highly efficient psb strains, h22, y11, y14, y34, and s32, were screened and isolated from an alfalfa rhizosphere in heavy metalcontaminated. Isolation and identification of phosphate solubilizing. Potassium is a vital component of plant nutrition package limiting crop yield and. A study was conducted to isolate phosphatesolubilizing bacteria psb from aerobic rice grown in penang malaysia and to determine some biochemical properties of the isolates such as, organic acids, enzymes, indoleacetic acid iaa, siderophore production and its antagonistic effect against pathogen rhizoctonia solani. The aim of the current study was to identify efficient phosphofungi that could be developed as commercial microbial agents. Isolation and molecular characterization of phosphate. Screening, isolation, biochemical and plant growth. Isolation and characterization of phosphatesolubilizing. Isolation, characterization and identification of potassium solubilizing bacteria from rhizosphere soil of maize zea mays.
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